RESPONSE OF TOMATO PLANTS EXPOSED TO TREATMENT WITH NANOPARTICLES

Tommaso Giordani; Alberto Fabrizi; Lucia Guidi; Lucia Natali; Giulia Giunti; Fausto Ravasi; Andrea Cavallini; Alberto Pardossi

doi:10.6092/issn.2281-4485/3748

Authors

Tommaso Giordani Dipartimento di Biologia delle Piante Agrarie, University of Pisa, Pisa
Alberto Fabrizi Dipartimento Tecnica e Gestione dei Sistemi Industriali, University of Padova, Padova
Lucia Guidi Dipartimento di Biologia delle Piante Agrarie, University of Pisa, Pisa
Lucia Natali Dipartimento di Biologia delle Piante Agrarie, University of Pisa, Pisa
Giulia Giunti Dipartimento di Biologia delle Piante Agrarie, University of Pisa, Pisa
Fausto Ravasi Dipartimento di Biologia delle Piante Agrarie, University of Pisa, Pisa
Andrea Cavallini Dipartimento di Biologia delle Piante Agrarie, University of Pisa, Pisa
Alberto Pardossi Dipartimento di Biologia delle Piante Agrarie, University of Pisa, Pisa

DOI:

https://doi.org/10.6092/issn.2281-4485/3748

Keywords:

nanoparticles, tomato, hydroponic, root uptake

Abstract

In this work the response of Tomato plants cv. Micro-Tom to nanoparticles (NPs) treatment was investigated. Tomato seedlings were grown in hydroponic condition and NPs treatments were carried out by adding Fe₃O₄ or TiO₂NPs to nutrient solution. At the end of treatments, NPs root uptake and tissue deposition were investigated using Environmental Scanning Electron Microscope, equipped with energy dispersive spectroscopy for chemical identification. At morphological level, one week after the beginning of NP treatment, seedlings grown with high concentration of TiO₂ NPs showed an abnormal proliferation of root hairs, as compared to the control seedlings and to the seedlings exposed to Fe₃O₄NPs, Shoot morphology did not differ in tomato seedlings grown under different conditions and no symptoms of toxicity were observed in NP-treated plants. In order to analyse genetic effects of NPs treatments, RNA transcription was studied in roots of NP-exposed and control plants by Illumina RNA sequencing, evidencing the induction of transposable elements.

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RESPONSE OF TOMATO PLANTS EXPOSED TO TREATMENT WITH NANOPARTICLES

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