RESPONSE OF SOIL MICROBIAL BIOMASS TO CeO2 NANOPARTICLES
DOI:
https://doi.org/10.6092/issn.2281-4485/3829Keywords:
nanoparticles, cerium oxide, bioindications, soil microbial biomass, electron microscopyAbstract
Aim of this work was to assess the impact of the chronic exposure of CeO2 nanoparticles (NPs) (50 to 105 nm nominal size) on soil microbial biomass.
To evaluate if the CeO2 NPs can affect the soil quality, they were mixed to an A1 and A2 horizon of Epileptic Cambisols at a concentrations of 100 ppm and incubated in lab for short and medium (7 and 60 days) times, at a constant temperature (25°C) and moisture (60% WHC).
The preliminary results of the soil physicochemical analyses have showed an insolubility of the CeO2 NPs at short-term incubation in water, EDTA and aqua regia. The biological assays detect a storing of Ce-CeO2 in the microbial biomass at short time that decreases in the C amount. An increment of the basal respiration and a decrease in the amount of carbon soil microbial biomass determined a higher metabolic quotient (qCO2) than the control test, that identifies a stressful situation, most evident in the short term condition.
Physical-chemical characterization of the CeO2 NPs and of the soil before and after the NPs addition, was carried out by means of Environmental Scanning Electron Microscope (ESEM) and an Energy Dispersive Spectroscopy (EDS). The investigations showed Ce-NPs and Ce-compounds in both- incubation-condition samples. The control soil showed the presence of cerium associated with other elements, like P, Nd, La, Th e Si. From literature, it appears that these elements identify Monazite-Ce/Nd minerals, whose chemical formulas are respectively (Ce, La, Nd, Th) PO4 and (Nd, Ce, La) (P, Si) O4. The presence of CeO2 NPs was clearly detected in soil and recognized by ESEM morphological observations coupled with EDS characterization. The NPs chemical composition appears unaltered, while the size can be modified by NPs aggregation and clustering.
The results contribute to setting reference baseline values of cerium in soil and indicate an impact on the amount of carbon soil microbial biomass due to a higher metabolic quotient (qCO2) that can condition the soil fertility.
References
ANDERSON T.H., DOMSCH K.H. (1993) The metabolic quotient for CO2 (qCO2 ) as a specific activity parameter to assess the effects of environmental conditions, such as pH, on the microbial biomass of forest soils. Soil Biology & Biochemistry 25:393–395.
BEKYAROVA E., FORNASIERO P., KAŠPAR J., GRAZIANI M. (1998) CO oxidation on Pd/CeO2±ZrO2 catalysts. Catalysis Today 45:179-183.
BRANUM M.E., QUE JR. L. (1999) Double-strand DNA hydrolysis by dialnthanide complexes. Journal of Biological Inorganic Chemistry 4:593-600.
BROOKES P.C., LANDMAN A., PRUDEN G., JENKINSON D.S. (1985) Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method for measuring microbial biomass nitrogen in soil. Soil Biology & Biochemistry 17:837-842.
BROOKES PC. (1995) The use of microbial parameters in monitoring soil pollution by heavy metals. Biology and Fertility of Soils 19:269-279.
CLEVELAND C.C., LIPTZIN D. (2007) C:N:P stoichiometry in soil: is there a “redfield ratio” for the microbial biomass. Biogeochemistry 85:235-252.
COLEMAN J.G., JOHNSON D.R., STANLEY J.K., BEDNAR A.J., WEISS C.A., BOYD, R.E., STEEVENS J.A. (2010) Assessing the fate and effects of nano aluminum oxide in the terrestrial earthworm, Eisenia fetida. Environmental Toxicology & Chemistry 29:1575–80. CHRISTIAN P., VON DER KAMMER F., BAALOUSHA M., HOFMANN T. (2008) Nanoparticles: Structure, properties, preparation and behavior in environmental media. Ecotoxicology 17:326–343.
DILLY O., MUNCH J.C. (1998) Ratios between estimates of microbial biomass content and microbial activity in soils. Biology and fertility of Soils, 27:374-379.
FANG J., SHIAN X., WEN B., LIN J., OWENSG. (2009) Stability of titania nanoparticles in soil suspensions and transport in saturated homogeneous soil column. Environmental pollution 157:1101-1109.
GILLER K.E., WITTER E., McGRATH S.P. (1998) Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: a review. Soil Biology &
Biochemistry 30:1389–1414.
HAY S., SLAVEYKOVA V.I., PAYET J. (2007) Terrestrial ecotoxicity and effect factors of metals in life cycle assessment (LCA). Chemosphere 68:1489-1496.
HU C.W., LI M., CUI Y.B., LI D.S., CHEN J., YANG L.Y. (2010) Toxicological effects of TiO2 and ZnO nanoparticles in soil on earthworm Eisenia fetida. Soil Biology &
Biochemistry 42:586–91.
LEITA L., DE NOBILI M., MUHLBACHOVA G., MONDINI C., MARCHIOL L., ZERBI G., (1995) Bioavailability and effects of heavy metals on soil microbial biomass serviva
during laboratory incubation. Biology and Fertility of Soils 19:103-108.
JENKINSON D.S., BROOKES P.C., POWLSON D.S. (2004) Measuring soil microbial biomass. Soil Biology & Biochemistry 36:5–7.
JOERGENSEN R.G., MUELLER T. (1996) The fumigation-extraction method to estimate soil microbial biomass: calibration of the kEN value. Soil Biology &
Biochemistry 28:33-37.
KATADA H., SEINO H., MIZOBE Y., SUMAOKA J., KOMIYAMA M. (2008) Crystal structure of Ce (IV)/dipicolinate complex as a catalyst for DNA hydrolysis. Journal of
Biological Inorganic Chemistry 13:249-255.
KHAN K.S., HEINZE S., JOERGENSEN R.G. (2009) Simultaneous measurements of S. macronutrients. and heavy metals in the soil microbial biomass with CHCl3
fumigation and NH4NO3 extraction. Soil Biology & Biochemistry 41:309-314.
KLAINE S.J., ALVAREZ P.J.J., BATLEY G.E., FERNANDES T.F., HANDY R.D., LYON D.Y., MAHENDRA S., MCLAUGHLIN M.J., LEAD J.R. (2008). Nanomaterials in the
environment: Behavior, fate, bioavailability and effects. Environonmental Toxicology Chemestry 27:1825–1851.
KRETZSCHMAR R., BARMETTLER K., GROLIMUND D., YAN Y., BORKOVEC M., STICHER H. (1997) Experimental determination of colloid deposition rates and collision
efficiencies in natural pour media. Water Resources Research 33:1129-1137.
KUZMAN K.A.D., FINNEGAN M.P., BANFIELD J.F. (2006) Influence of surface potential on aggregation and transport of titania nanoparticles. Environmental Science
& Technology 40:7688-7693.
MCDONALD J.W., GHIO A.J., SHEEHAN C.E., BERNHARDT P.F., ROGGLI V.L. (1995) Rare earth (cerium oxide) pneumoconiosis: analytical scanning electron
microscopy and literature review. Modern Pathology 8:859–865.
NAVROTSKY A. (2004) Encyclopedia of Nanoscience and Nanotechnology. Marcel Dekker, Inc.
NAKAGAWA K., MURATA Y., KISHIDA M., ADACHI M., HIRO M., SUSA K. ( 2007) Formation and reaction activity of CeO2 nanoparticles of cubic structure and various
shaped CeO2-TiO2 composite nanostructures. Materials Chemistry and Physics104:30–39.
PAIRON J.C., ROOS F., IWATSUBO Y., JANSON X., BILLON-GALLAND M.A., BIGNONO J., BROCHARD P. (1994) Lung retention of cerium in humans. Occupational and
Environmental Medicine 51:195–199.
PARK B., MARTIN P., HARRIS C., GUEST R., WHITTINGHAM A., JENKINSON P., HANDLEY J. (2007) Initial in vitro screening approach to investigate the potential
health and environmental hazards of EnviroxTM – a nanoparticlulate cerium oxide diesel fuel additive. Particle and Fibre Toxicology 4, 12.
PANKHURST C.E., HAWKE B.G., MCDONALD H.J., KIRKBY C.A., BUCKERFIELD J.C., MICHELSEN P., O’BRIEN K.A., GUPTA V.V.S.R, DOUBE B.M. (1995) Evaluation of soil
biological properties as potential bioindicators of soil health. Australian Journal of Experimental Agriculture 35:1015–1028.
PINZARI F., TRINCHERA A., BENEDETTI A., SEQUI, P. (1999) Use of biochemical indices in the Mediterranean environment: comparison among soil and different
forest vegetation. Journal od Microbiological Method 36:21-28.
POWLSON D.S., BROOKES P.C., CHRISTENSEN B.T. (1987) Measurement of soil microbial biomass provides an early indication of changes in total soil organic
matter due to straw incorporation. Soil Biology & Biochemistry 19:159–164.
QUIK J.T.K., LYNCH I., VAN HOECKE K., MIERMANS C.J.H., DE SCHAMPHELAERE K.A.C., JANSSEN C.R., DAWSON K.A., COHEN STUART M.A., VAN DE MEENT D. (2010)
Effect of natural organic matter on cerium dioxide nanoparticles settling in model fresh water. Chemosphere 81:711-715.
RAWLINGS J., CLELAND W.W., HENGGE A.C. (2003) Metal ion catalyzed hydrolysis of ethyl p-nitrophenyl phosphate. Journal Inorganic Biochemistry 93:61- 65.
SATO T., KATAKURA T., YIN S., FUJIMOTO T., YABE S. (2004) Synthesis and UV-shielding properties of calcia-dopedCeO2 nanoparticles coated with amorphous
silica. Solid State Ionics 172:377–382.
SOUVÉ S., HENDERSHOT W., ALLEN H. (2000) Solid-liquid partitioning of metals in contaminated soil: dependence on pH. total metal burden. and organic matter.
Environmental Science &Technology 34:1125-1131.
STOIMENOV P.K., KLINGER R.L., MARCHIN G.L., KLABUNDE K.J. (2002) Metal oxide nanoparticles as bactericidal agents. Langmuir 18:6679–6686.
TAN F., ZHANG Y., WANG J., WIE J., CAI Y., QIAN X. (2008) An efficient method for dephosphorylation of phosphopeptides by cerium oxide. Journal of Mass
Spectrometry 43: 628-632.
TANG Z., WU L., LUO Y., CRISTIE P. (2009) Size fractionation and characterization of nanocolloidal particles in soils. Environmental Geochemical Health 31:1–10
THILL A., ZEYONS O., SPALLA O., CHAUVAT F., ROSE J., AUFFAN M., FLANK A.M. (2006) Cytotoxicity of CeO2 nanoparticles for Escherichia coli. Physico-chemical
insight of the cytotoxicity mechanism. Environmental. Science. Technology 40:6151–6156.
TRIERWEILER J.F., LINDSAY W.L. (1969) EDTA-ammonium carbonate soil test for Zn. Soil Science Society of America Proceeding 33:49-54.
VAN HOECKE K., QUIK J.T.K., MANKIEWICZ-BOCZEK J., DE SCHAMPHELAERE K.A.C., ELSAESSER A., VAN DER MEEREN P., BARNES C., MCKERR G. , HOWARD C.V., VAN
DE MEENT D., RYDZYŃSKI K., DAWSON K.A. , SALVATI A., LESNIAK A., LYNCH I., SILVERSMIT G., DE SAMBER B. , VINCZE L., JANSSEN C.R. (2009) Fate and effects of
CeO2 nanoparticles in aquatic ecotoxicity tests, Environmental Science and Technology 43:4537-4546.
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