Harnessing heavy metal-tolerant bacteria and phytotoxicity assessment for ecofriendly treatment of industrial effluents
DOI:
https://doi.org/10.6092/issn.2281-4485/19182Keywords:
Eco friendly, Industrial wastewater, Heavy metals, Microbial Bioremediation, PhytotoxicityAbstract
This study treated heavy-metal pollution in industrial wastewater by isolating and characterizing heavy metal-tolerant bacterial strains for bioremediation of industrial wastewater. The effluent samples were sterilized and aseptically inoculated with isolated heavy metal-tolerant bacteria such as Bacillus lichniformis, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. The samples were subjected to physicochemical analysis, and titrimetric analysis. Further, Atomic Absorption Spectroscopy (AAS) was used for Heavy metal analysis. Phytotoxic assay was carried out by seed germination tests and pot culture study. Over 96-hour period, improvement featuring reduced turbidity, a stabilized neutral pH, and a substantial 72% decrease in total solids was observed. The application of the bioremediation techniques using heavy metal tolerant bacteria resulted in a significant reduction of heavy metal concentrations, surpassing 85%. Moreover, seed germination rates in the treated industrial effluent were higher than the untreated samples. Pot culture tests further revealed diminished toxicity (2.5%) in treated roots compared to untreated ones (36.7%), accompanied by increased root and shoot growth in the treated pots. The noticeable enhancement in germination rates and the improved growth of plants in the treated effluent underscore the promising potential for the sustainable application of these eco-friendly bacteria, making them a cost-effective microbial bioremediation solution for industrial wastewater.
References
AYAZ T., KHAN S., KHAN A.Z., LEI M., ALAM M. (2020) Remediation of industrial wastewater using four hydrophyte species: A comparison of individual (pot experiments) and mix plants (constructed wetland). Journal of Environmental Management, 255:109833. https://doi.org/10.1016/j.jenvman.2019.109833
AZZAM A.M., TAWFIK A. (2015) Removal of heavy metals using bacterial bio-flocculants of Bacillus sp. and Pseudomonas sp. Journal of Environmental Engineering and Landscape Management, 23 (4):288–294. https:// doi.org/10.3846/16486897.2015.1068781
HOBMAN J.L., BROWN N.L. (1996) Overexpression of MerT, the mercuric ion transport protein of transposon Tn501, and genetic selection of mercury hypersensitivity mutations. Molecular and General Genetics, 250 (1):129–134. https://doi.org/10.1007/BF02191833
IANEVA O.D. (2009) Mechanisms of bacteria resistance to heavy metals. Mikrobiolohichnyi Zhurnal (Kiev, Ukraine), 71(6): 54–65. https://pubmed.ncbi.nlm.nih. gov/20455433/
IWASAKI Y., KAGAYA T., MIYAMOTO K., MATSUDA H. (2009) Effects of heavy metals on riverine benthic macroinvertebrate assemblages with reference to potential food availability for drift‐feeding fishes. Envi-ronmental Toxicology and Chemistry, 28(2):354–363. https://doi.org/10.1897/08-200.1
JI G., SILVER S. (1955) Bacterial resistance mechanisms for heavy metals of environmental concern. Journal of Industrial Microbiology, 14(2):61–75. https://doi.org/ 10.1007/BF01569887
MANISHA N, DINESH S, ARUN K. (2011) Isolation and characterisation of bacteria resistant to heavy metals Cadmium (Cd), Arsenic (As), Mercury (Hg) from indu-strial effluent. Global Journal of Applied Environmental Sciences, 1(2):127–132. https://www.ripublication.com/ gjaes/gjaesv1n202.pdf
SAMADI M.T., SADEGHI S., RAHMANI A., SAGHI M.H. (2015) Survey of Water Quality in Moradbeik River Basis on WQI Index by GIS [Internet]. Rochester, NY. https://papers.ssrn.com/abstract= 2610038
TAYANG A., SONGACHAN L.S. (2021) Microbial Bioremediation of Heavy Metals. Current Science 120(6):
YUMPU.COM. (2023) Biosorption of Heavy Metals from Aqueous Solution using Bacillus Licheniformis. Avai lable from: https://www.yumpu.com/en/document/ view/53787901/biosorption-of-heavy-metals-from-aqueous-solution-using-bacillus-licheniformis
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Copyright (c) 2024 Sharmila Shashikant Ghangale, Ramling Sidaramappa Saler, Sharad Ratan Khandelwal, Dilip Vishwanath Handore, Anita Vishwanath Handore
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