The biodegradation of low-density polyethylene by Bacillus species

Authors

  • Felicia E. Uwakwe Department of Environmental Health Science, Federal University of Technology, Owerri, Nigeria
  • Tobias I.N. Ezejiofor Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
  • Ernest A.A. Anyalogbu Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
  • Toochukwu E. Ogbulie Department of Biotechnology, Federal University of Technology, Owerri, Nigeria

DOI:

https://doi.org/10.6092/issn.2281-4485/20474

Keywords:

polyethylene, biodegradation, Bacillus spp., eco-friendly, dumpsite

Abstract

Polyethylene (PE) products have short-term applications, resulting in the daily release of enormous quantities of plastic wastes into the environment with their attendant public and environmental health threats of global concern. Developing an eco-friendly management method to abate the menace prompted this research work. Four Bacillus species were isolated from different dumpsites in Owerri Metropolis, and identified using 16S rDNA sequencing, as B.pumilus, B.siamensis, B.coagulans and B. subtilis. The ability of the microbial isolates to degrade polyethylene (table water sachets) was determined using microbial growth measurement in liquid medium (Optical Density (OD600)), disappearance and introduction of functional groups (Fourier Transform Infrared spectroscopy (FTIR) and weight loss analysis measured after sixty (60) days of incubation in mineral salt vitamin media. Microbial density showed a gradual and progressive increase from 0 day to 60 days : B. siamensis (0.12 - 0.84), B.pumilus (0.11-.67), B.coagulans (0.12 - 0.61) and B.subtilis (0.11 – 0.60), as against the control medium which remained unchanged (0.11). Similarly, FTIR spectra showed additional functional groups (carboxylic acids, ethers, esters, amines, methylene, alcohols, and alkenes in the polyethylene materials exposed to the test isolates: B.siamensis (17 peaks), B.pumilus (12 peaks), B.coagulans and B.subtilis (13 peaks each), but control (8 peaks). There were subatantial reduction in the residual weights of the PE materials: B.siamensis (52%), B.pumilus (26%), B.coagulans and B.subtilis (18% each). The observations showed that these Bacillus spp. indigenous to Owerri Metropolis have the ability to degrade PE and utilize it as a carbon source, and it was concluded that biodegradation was achieved, and that PE which was regarded to be inert can be biodegraded when exposed to the right microorganisms.

References

ALI S.S., ELSAMAHY T., AL-TOHAMY R., ZHU D., MAHMOUD Y.A., KOUTRA E., METWALY M.A. (2021). Plastic Waste Biodegradation: Mechanism, Challen-ge and Future Prospects. Science of the Total Environ-ment,780. http://doi.org/10.1016/j.scitotenv.2021.149712

ARUTCHELVI J., SUDHAKAR M. ARKATKAR A., DOBLE M., BHADURI S., UPPARA P.V. (2008) Biodegradation of polyethylene and polypropylene. Indian Journal of Biotechnology, 7:9-20.

ASM - American Society of Mrcrobiology (2020) Identifying bacteria through look, growth, stain and strain. retrieved: http://www.asm.org/article

AZEKO S.T., ETU-UDO G.A., ODUSANYA O.S. MALATESTA K., ANUKU N., SOBOYEJO W.O. (2015) Biodegradation of Linear Low Density Polyethylene by SerratiaMarcescens subsp. marcescens and its Cell Free Extracts. Waste Biomas Valor, 6:1047-1057. https://doi.org/10.1007/S12649-015-9421-0.

AZIZA A., FAIRUS S., SAARI (2024) Isolation and cha-racterization of polyethylene and polyethylene teraphthalate -degrading bacteria from Jakarta, Indonesia. The Open Biotechnology Journal,18:e187400787280343 http://dx.doi.org/10.2174/0118740707280343231208102253

BARDAJI D.K.R., FURLAN J.P.R., SHEHLING E.G (2019) Isolation of a Polyethylene Degrading Paenibacillius sp. from a landfill in Brazil. Archives of Microbiology, 201: 699-704. https://doi.org/10.1007/S00203-019-01637-3.

BHARDWAJ H., GUPTA R., TIWARI A. (2012) Micro-bial Population Associated with Plastic Degradation. Open Access Scientific Reports, 1(5):272-274. http://dx.doi.org/10.4172/scientificreport.272

BIKI S.P., MAHMUD S., AKHTER S., RAHMAN M.J., RIX J.J., AL BACHCHU M.A., AHMED N. (2021) Polye-thylene degradation by Ralstonia sp. strain SKM2 and Ba-cilliussp strain SMI isolated from landfill soil site. Environ-mental Technology and Innovation, 22. http://doi.10.1016/j.eti.2021.101851.

BRANDSON A.M., GARCIA A.M., KHLYSTOV N.A., WU W.M., CRIDDLE C.S. (2021) Enhanced bioavailabi-lity and degradation of polystyrene in an enrichment deri-ved from the gut microbiome of Tenebrio molitor(meal worm larva). Environmental Science and Technology, 53 (3):2027–2036. https://doi.org/10.1021/ace.est.0c04952

CHAMAS A., MOON H., ZENG J.J., QUI Y., TABA-SSUM T., JANG J.H., ABU-OMAR M., SCOTT S.L., SUH S. (2020) Degradation rates of plastic in the environ-ment. ACS Sustainable Chemistry and Engineering, 8:3494 -3511. https://doi.org/10.1021/acssuschememg.9b06635

CHEESBROUGH M. (2006) Biochemical tests to identify bacteria. In: Cheesbrough M. (ed). District laboratory practice in tropical countries, part 2, 2nd Edition. Cambridge University Press, UK, pp 62-70.

CHEN M., LIU Z., JIANA S., HOU H. (2020) A feasible route for reutilization of plastic wastes. Science of Total Environment, 710. http://doi.org/10.1016/j.scitotenv.2020.136256

DEVI D., GUPTA K.K., CHANDRA H., SHARMA K.K., SAGA K., MORI E., MALA DE FARIA (2023) Biodegradation of low-density polyethylene (LDPE) through application of indigenous strain Alicalinenes faecalis ISJ128.Environmental Geochemistry and Health. 45(12): 9391-9401 https://doi.org/10.1007/s10652-023-01590-z

EMMANUEL-AKERELE H.A., AKINYEMI P.O., IGBOGBO-EKPUNOBI O.E. (2022) Isolation and identification of plastic degrading bacteria from Dumpsites – Lagos. Advances in Environmental Technology, 1:59– 71. https://doi.org/10.22104/AET.2020.5268.1428

ESMAEILI A., POURBABAEE A.A., ALIKHANI H.A., SHABANI F.,ESMAEILI E. (2013) Biodegradation of low densitypolyethylene (LDPE) by mixed culture of Lynsiniba-cillusxylanilyticus and Aspergillus nigerin soil. PLOS ONE, 8(9):1-10. https://doi.org/10.1371/journal.pone.0071720

GILAN I., HADAR Y., SIVAN A. (2004) Colonization, biofilm formation and biodegradation of polyethylene by a https:/strain of Rhodococcusrubber. Applied Microbiology and Biotechnology, 65:97-104. https://doi.org/:10.1007/500253-004-1584-8 04

HADAD D., GERESH S., SWAN A. (2005) Biodegradation of polyethyelene by the thermophilic bacteriumBrevibacillusborstelensis. Journal of Applied Microbiology, 98:1093 -1100. https://doi.org/10.1111/j.1365-2672-2005.02553.x

HAJRAMURNI A. (2018) Indonesia aims to tackle plastic waste. www.thejakartapost.com

IBIENE A.A., STANLEY H.O., IMMANUEL O.M. (2013) Biodegradation of polyethylene by Bacillus sp. Indigenous to the Niger Delta Magrove Swamp. Nigerian Journal of Biotechnology, 26: 68-79. ISSN: 0189 17131

JAINA A., PANIGRAHY S.S., DONGRE N., JAIN P. (2023) Isolation and Characterization of Plastic-Degrading Bacteria from the Soil. Scope, 13(4): 838-842. https://7d17c-838-842.202317754

JANDA J.M., ABBOTT S.L. (2007) 16S rRNA Gene sequencing for bacterial identification in the diagnostic laboratory. Journal of Clinical Microbiology, 45(9):2761-2764.

KHANDARE S.D., CHAUDHARY D.R., JHA B. (2021) Marine bacterial biodegradation of low-density polyethylene (LDPE) plastic. Biodegradation, 32(2):127-143. https://doi.org/10.1007/51032-021-09927-0

KOPECKA R., KUBINOVA I., SOVOVA K., MRAVCOVA L., VITEZ T., VITEZOVA M. (2022) Microbial degradation of virgin polyethylene by bacteria isolated from a landfill site. SN Applied Sciences, 4:302-312. https://doi.org/10.1007/s42452-022-05182-x

KUBOWICZ S., BOOTH A.M. (2017) Biodegradability of plastics: Challenges and Misconception. Environmental Science and Technology, 51(21):12056 -12060. https://doi.org/10.1021/ace.est.7b04051

LIN Z., JIN T., ZOU T., XU L., XI B., XU D., HE J. (2022). Current Process on Plastic/Microplastic Degrada-tion: Fact influences and mechanism. Environmental Pollu-tion, 304:119-159. doi:10.1016/j.envpol.2022.119159

LONGO C.,SAVARIS M., ZENI M., NICHELE R.B., COULON GRISA A.M. (2011) Degradation study of polypropylene (pp) and bioriented polypropylene (BOPP) in the environment. Materials Research, 14(4). http://dx.doi.org/10.1590/51516-14392011005000080

MUKHERJEE S., ROYCHAUDHURU U., KUNDU P.P (2018) Biodegradation of polyethylene via complete solubilization by the action of Pseudomonas fluorescence, biosurfactant produced by Bacillius licheniformis and anionic surfactant. J. Chem. Technology Biotechnology, 93: 1300-1311. http://doi.org/10.1002/jctb.5489

NAG M., LAHIRI D., DUTTA B., JADAV G., RAY R.R. (2021) Biodegradation of used polyethylene bags by a new marine strain of Alcaligenes faecalis LNDR-1. Environmental Science Pollution Researc International, 28(30):41365-41379. DOI:10.1007/S11356-021-13704-0

NANDA S. SAHU S.S., ABBRAHAM J. (2010) Studies on the biodegradation of natural and synthetic polyethylene by Pseudomonas spp. Journal of Applied Science and Envi-ronmental Management, 14(2):57-60. ISSN:1119-8362.

NI Z., MINGZHU D., YINGJIN Y. (2022) Current Advances in Biodegradation of polyolefins. Micro orga-nisms, 10(8):1537. https://doi.org/10.3390/microorganisms.100081537

NOVOTNY C., MALACHOVA K., ADAMUS G., KWIEDIEN M., LOTTI N., SOCCIO M., VERNEY V., FAVA F. (2018) Deteriotation of irradiation/high-tempera-ture pretreated Linear Low- Density PE (LLDPE) by Bacilliusamyloliquefaciens. International Journal of Biode-gradation 132:259-267. https://doi.org/:10.1016/j.ibiod.2018.04.014

OGBULIE T.E., NWAKANMA C. (2015) Essential Analytical Methods in Biotechnology. Unique Books Publisher, Nigeria. pp. 63-89.

PATIL R., BAGDE U.S. (2015) Enrichment and isolation of microbial strains degrading bioplastic polyvinyl alcohol and time course study of their degradation potential. African Journal of Biotechnology. 14(27):2216-2226. https://doi.org/10.5897/AJB2011.3980.

PRAJAPATI R., KHOLI K., MAITY S., SHARMA B.K (2021) Potential chemicals from plastics waste. Molecules, 26(11):3175. https://doi.org/10.3390/molecules26113175

REN L., MEN L., ZHANG Z., GUAN F., TIAN J., WANG B., WANG J., ZHANG Y., ZANG W. (2019) Biodegradation of Polyethylene by Enterobacter sp. D1 from the Gut of Wax Moth Galleria mellonella. Interna-tional Journal of Environmental Research and Public Health,16:1941. https://doi.org/10.3390/ijerph16111941

SAMBROOK J., RUSSELL D.W (2001) Molecular cloning: A laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, NY, USA. SKARIYACHAN S., SETTUR A.S., NAIK S.Y., NARK A.A, USHARAN M.,VASIST K.S. (2017) Enhanced biodegradation of low and high-density PE by novel bacterial consortium formulated from plastic contaminated cow dung under thermophiolic conditions. Envionmental Science and Pollution Research 24:8443-8457. https://doi.org/10.1007/s11356-017-85370

SKARIYACHAN S., PATIL A.A, SHANKAR A., MANJUANTH M., BACHAPPANAVAR N., KIRAN S. (2018) Enhanced polymer degradation of polyethylene and polypropylene by novel thermophilic consortia of Breviba-cilliussps. and Aeurinibacillius sp. screened from waste management landfills and sewage treatment plants. Polymer Degradation stability 149:52-68. https://doi.org/10.1016/j.polymdegradstab.2018.018

SRIKANTH M., SANDEEP T.S.R.S., SUCHANTHA K.K., GODI S. (2022) Biodegradation of plastic polymers by fungi: A brief review. Bioresources and Bioprocessing, 9(42). https://doi.org/10.1186/540643-022-00532-4

UGUERI U., ATUNANYA E.I., USMAN Z. (2022) Biodegradation of polystyrene plastics by bacterial isolation from plastic composted waste oil and molecular charactyerization of plastic degrading bacterial isolates. African Journal of Microbiology Research, 16(6).247-257. https://doi.org/10-5897/AJMR2022.9341

UWAKWE F.E., EZEJIOFOR T.I.N., OGBULIE T.E., ANYALOGBU E.A.A., OKAFOR S.A. (2023) Investi-gating the Biodegradation of Low-Density Polyethylene by Proteus and Serratia Spp. Engineering and Technology Journal, 8(3):2052-2055. doi: https://doi.org/104719/etj/v8i3.08

UWAKWE F.E., EZEJIOFOR T.I.N., OGBULIE T.E., ANYALOGBU E.A.A. (2023) A Comparative Study of Biodegradation of Low-Density Polyethylene by Proteus and Lactobacillus Spp. International Journal of Advanced Research in Biological Sciences, 10(7): 93 – 102. https://doi.org/10.1007/s42770-024-01487-8

YOON M.G., JEON H.J., KIM M.N. (2012) Biode-gradation of polyethylene by a soil bacterium and alkb cloned recombinant cell. Journal of Bioremediation and Biodegradation 3(145). https://doi.org/10.4172/2155-6199-.1000145

ZHANG Y., PENDERSON J.N., ESER B.E., GUO, Z.(2022) Biodegradation of polyethylene and polystyrene: From microbialdeteroriation to enzyme discovery. Journal of Biotechnology Advances, 60:107991. https://doi.org/10.1016/j.biotechadv.2022.107991

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Published

2025-02-03

How to Cite

Uwakwe, F. E., Ezejiofor , T. I., Anyalogbu , E. A., & Ogbulie , T. E. (2025). The biodegradation of low-density polyethylene by Bacillus species. EQA - International Journal of Environmental Quality, 67, 26–35. https://doi.org/10.6092/issn.2281-4485/20474

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