Health risk assessment of heavy metals in Cassava cultivated on leachate-contaminated soil during the early transition from landfilling to co-landfilling with incineration
DOI:
https://doi.org/10.6092/issn.2281-4485/21367Keywords:
Health risk assessment, Heavy metal, Cassava, soil, LandfillAbstract
This study aimed to investigate heavy metal contamination in soil and cassava in the area of the Khon Kaen Municipality landfill during the transition period of waste management (December 2015), focusing on non-carcinogenic and carcinogenic health risks from consuming cassava tubers. Soil (0-30 cm) and cassava samples were collected from agricultural areas most affected by leachate leakage. Soil and cassava samples were digested for heavy metal analysis using microwave-assisted acid digestion (USEPA 3052), followed by heavy metal analysis with ICP-AES (USEPA 6010D). The analysis revealed that the concentration of heavy metals in soil did not exceed the WHO/FAO permissible limits, but contamination levels in cassava tubers for Cd, Cr, and Pb exceeded the permissible limits. Cassava demonstrated the ability to accumulate heavy metals in its tubers, with Ni showing the highest bioaccumulation potential (BCF = 5.837). Once accumulated in tubers, heavy metals translocated to leaves (Cd, Cr, Fe, Mn, Pb, Zn) and stems (Cr, Fe, Mn, Pb, Zn), with Mn exhibiting the highest translocation potential (TF tuber to leave = 10.670, TF tuber to stem = 7.094). Health risk assessments showed that both non-carcinogenic and carcinogenic health risks for both children and adults were unacceptable (HI >1, TCR >10-4). These findings highlight that, during the initial phase of waste management improvements, contamination persists in the soil and cassava, posing health risks to consumers. Therefore, enhancing leachate management systems is essential, and the results should serve as a reference for future waste management.
References
ABDULLAH N.H., KEAN O.B., HIRMIZI N.M., YUSOFF N., MOHD A.R.N. (2020) Method Validation of Heavy Metals Determination in Traditional Herbal Tablet, Capsule and Liquid by Graphite Furnace Atomic Absorption Spectrometer and Flow Injec-tion for Atomic Spectroscopy Hydride System. Asian J. Pharmacogn, 4(3):37-45.
ALADESANMI O.T., OROBOADE J.G., OSISIOGU C.P., OSEWOLE A.O. (2019) Bioaccumulation Factor of Selected Heavy Metals in Zea mays. Journal of health & pollution, 9(24):191207. https://doi.org/10.5696/2156-9614-9.24.191207
AOAC (2002) AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals. AOAC, Washington, DC, USA.
ARIVALAGAN P., CHANDRAMOHAN G., ASHUTOSH S. (2024) Heavy metal accumulation in root and shoot tapioca plant biomass grown in agriculture land situated around the magnesite mine tailings. Environmental Research, 257:119287. https://doi.org/10.1016/j.envres.2024.119287
ASIAN DEVELOPMENT BANK (2015) THAILAND Industrialization and economic catch-up. ADB, Manila, Philippines.
AVILA P.F., FERREIRA S.E., CANDEIAS C. (2017) Health risk assessment through consumption of vegetables rich in heavy metals: the case study of the surrounding villages from Panasqueira mine, Central Portugal. Environmental Geochemistry and Health, 39:565–589.
BANYAM B. (2017) Contamination of Heavy Metals in Leachate, Groundwater and Soil at Khon Kaen Municipal Solid Waste Landfill, Khon Kaen Province. B.Sc. Thesis, Department of Earth Science, Faculty of Science, Kasetsart University, Thailand.
BORTEY-SAM N., NAKAYAMA S.M.M., AKOTO O., IKENAKA Y., FOBIL J.N., BAIDOO E., MIZUKAWA H., ISHIZUKA, M. (2015) Accumulation of Heavy Metals and Metalloid in Foodstuffs from Agricultural Soils around Tarkwa Area in Ghana, and Associated Human Health Risks. International Journal of Environmental Research and Public Health, 12(8):8811-8827. https://doi.org/10.3390/ijerph120808811
CHINEDU E.I., EVANS C.N., JOHN D.N., ENOS I.E., TOCHI E.E., ROSELINE F.N.T., ONYENONACHI C.I., EJEAGBA O.I., IKENNA N.N., ETIENNE C.C., CHIJIOKE N.U., CHINOMSO E.E., NWAOGWUGWU N.U. (2021) Heavy metal contamination of Cassava (Manihot esculenta Crantz) grown on quarry soils in Umunneochi Abia State and its health implications. EQA - International Journal of Environmental Quality, 45:1-9. https://doi.org/10.6092/issn.2281-4485/12563
CHOPRA B.K., BHAT S., MIKHEENKO I. P., XU Z., YANG Y., LUO X., CHEN H., ZWIETEN L., LILLEY R.M., ZHANG R. (2007) The characteristics of rhizosphere microbes associated with plants in arsenic-contaminated soils from cattle dip sites. Science of the Total Environment, 378(3):331–342.
CSIRO - Commonwealth Scientific and Industrial Research Organization (2021) Soil sampling guidelines strenthening regional collaboration on soil analysis.
https://research.csiro.au/pacsoils/wp-content/uploads/ sites/404/2021/12/2-Soil-sampling-manual.pdf
DIKIOYE E.P., CHARITY E., KPOBARI W.N. (2018) Potential Human Health Risk Assessment of Heavy Metals via Consumption of Root Tubers from Ogoniland, Rivers State, Nigeria. Biological Trace Element Research, 186:568–578. https://doi.org/10.1007/s12011-018-1330-1
DRAGOVIĆ S., MIHAILOVIĆ N., GAJIĆ B. (2008) Heavy metals in soils: distribution, relationship with soil characteristics and radionuclides and multivariate assessment of contamination sources. Chemosphere, 72(3):491-495. https://doi.org/10.1016/j.chemosphere.2008.02.063
GARCÍA L.J., MÉNDEZ J., PÁSARO E., LAFFON B. (2010) Genotoxic effects of lead: an updated review. Environment international, 36(6):623–636. https://doi.org/10.1016/j.envint.2010.04.011
WHO (2023) Exposure to lead: a major public health concern. WHO, Geneva, Switzerland.
HOWLADAR M.F. (2017) An Assessment of surface water chemistry with its possible sources of pollution around the Barapukuria thermal power plant impacted area, Dinajpur, Bangladesh. Groundwater for sustainable development, 5:38−48.
IRIS (2006) Integrated Risk Information System 2006. https://iris.epa.gov/AtoZ/?list_type=alpha.
JIANG M., ZENG G., ZHANG C., MA X., CHEN M., ZHANG J., LU L., YU Q., HU L., LIU L. (2013) Assessment of Heavy Metal Contamination in the Surrounding Soils and Surface Sediments in Xia-wangang River, Qingshuitang District. PLoS ONE, 8(8):71176. https://doi.org/10.1371/journal.pone.0071176
JOMOVA K., ALOMAR S.Y., NEPOVIMOVA E., KUCA K., VALKO M. (2025) Heavy metals: toxicity and human health effects. Archives of toxicology, 99(1):153–209. https://doi.org/10.1007/s00204-024-03903-2
KAMAL U., MOHAMMAD A.A.G., MOHAMMED H.A.D. (2019) The assessment of cadmium, chromium, copper, and nickel tolerance and bioaccumulation by shrub plant Tetraena qataranse. Scientific Reports, 9:5658. https://doi.org/10.1038/s41598-019-42029-9
KHON KAEN PROVINCIAL STATISTICAL OFFICE (2020) Municipal Solid Waste Management Situation in Khon Kaen Province, 2019. Khon Kaen Provincial authority, Khon Kaen, Thailand. (In Thai)
KIM I.S., KANG K.H., GREEN J.P., LEE E.J. (2003) Investigation of heavy metal accumulation in Polygonum thunbergii for phytoextraction. Environmental Pollution, 126(2):235-243. https://doi.org/10.1016/S0269-7491(03)00190-8
KOVÁCS B.É., DOMOKOS E., BIRÓ B. (2021) Toxic metal phytoextraction potential and health-risk parameters
of some cultivated plants when grown in metal-contaminated river sediment of Danube, near an industrial town. Environ Geochem Health, 43:2317–2330. https://doi.org/10.1007/s10653-021-00880-8
LAND DEVELOPMENT DEPARTMENT OF THAILAND (2021) Land Use Zone for Economic Crops: Cassava. Land Development Department of Thailand, Bangkok, Thailand. (In Thai)
LATIF A., BILAL M., ASGHAR W., AZEEM M., AHMAD M.I. (2018) Heavy metal accumulation in vegetables and assessment of their potential health risk. J. Environ. Anal. Chem, 5(1):1000234.
LOCAL ADMINISTRATION DEPARTMENT OF THAILAND (2016) The “Thailand Zero Waste” Action Plan 2016-2017. Local Administration Department of Thailand and Pollution Control Department of Thailand, Bangkok, Thailand. (In Thai)
MÁRIO M., SÓNIA V.G., RUI S.O., CÉLIA M.M., ORLANDO A.Q. (2024) Assessment of heavy metals and human health risk associated with the consumption of crops cultivated in industrial areas of Maputo, Mozambique. Journal of Environmental Science and Health, Part A, 59(4):200-211.
https://doi.org/10.1080/10934529.2024.2349478
McGREGOR D.B., BAAN R.A., PARTENSKY C., RICE J.M., WILBOURN, J.D. (2000) Evaluation of the carcino-genic risks to humans associated with surgical implants and other foreign bodies - a report of an IARC Monographs Programme Meeting. International Agency for Research on Cancer. European journal of cancer (Oxford, England : 1990), 36(3):307–313.
https://doi.org/10.1016/s0959-8049(99)00312-3
NOURI J., KHORASANI N., LORESTANI B., KARAMI M., HASSANI A.H., YOUSEFI N. (2009) Accumulation of heavy metals in soil and uptake by plant species with phytoremediation potential. Environ Earth Sci, 59:315–323. https://doi.org/10.1007/s12665-009-0028-2
NTP-National Toxicology Program (2008). Toxicology and carcinogenesis studies of sodium dichromate dihydrate (Cas No. 7789-12-0) in F344/N rats and B6C3F1 mice (drinking water studies). National Toxicology Program technical report series, 546:1–192.
OMOBOLAJI O.A., MAUREEN O.U., ALEX I.A.F., TOOCHUKWU T.U., OKECHUKWU N.N.O., SUNDAY O.J., AUGUSTINE O.W. (2024) Assessment of heavy metal contents in farm produce around Ewekoro and its health implications on consumers. SN Appl. Sci., 5:340. https://doi.org/10.1007/s42452-023-05550-1
OGBONNA P.C., OSIM O.O., BIOSE E. (2020) Deter-mination of Heavy Metal Contamination in Soil and Accumulation in Cassava (Manihot Esculenta) in Automobile Waste Dumpsite at Ohiya Mechanic Village. NIJEST, 4(1):54-69.
OMOROGIEVA O.M., TONJOH J.A. (2020) Bioavaila-bility of heavy metal load in soil, groundwater, and food crops manihot esculenta and carica papaya in dumpsite environment. International Journal of Environmental Science and Technology, 17:4853-4864.
https://doi.org/10.1007/s13762-020-02812-y
ONYEDIKACHI U.B., BELONWU D.C., WEGWU M.O. (2018) Human health risk assessment of heavy metals in soils and commonly consumed food crops from quarry sites located at Isiagwu, Ebonyi State. Ovidius University Annals of Chemistry, 29(1):8 - 24.
ORISH E.E., HARRISON A.O., IFY L.N., ANTHONET N.E. (2019) Probabilistic health risk assessment of heavy metals in honey, Manihot esculenta, and Vernonia amygdalina consumed in Enugu State, Nigeria. Environ Monit Assess, 191(7):424. https://doi.org/10.1007/s10661-019-7549-2
PALASH K.D. ARIFA N., MOSUMMATH H.A. (2020) Health risks assessment of heavy metal contamination in drinking water collected from different educational institutions of Khulna city corporation, Bangladesh. Advances in Environmental Technology, 4:235-250. https://doi.org/10.22104/AET.2021.4932.1331
POLLUTION CONTROL DEPARTMENT OF THAILAND (2011) National 3Rs Strategy. Pollution Control Department of Thailand, Bangkok, Thailand. (In Thai)
POLLUTION CONTROL DEPARTMENT OF THAILAND (2022) Report on the Situation of Municipal Solid Waste Disposal Sites in Thailand in 2022. Pollution Control Department of Thailand, Bangkok, Thailand. (In Thai)
PRABHAT K.R., SANG S.L., MING Z., YIU F.T., KI H.K. (2019) Heavy metals in food crops: Health risks, fate, mechanisms, and management. Environment International, 125:365-385. https://doi.org/10.1016/j.envint.2019.01.067
PRANAV P.D., DEBLINA D. (2024) Landfill leachate a potential challenge towards sustainable environmental management. The Science of The Total Environment, 926(18):171668. https://doi.org/10.1016/j.scitotenv.2024.171668
QING L., XIAOHUI L., LEI H. (2022) Health risk assessment of heavy metals in soils and food crops from a coexist area of heavily industrialized and intensively cropping in the Chengdu Plain, Sichuan, China. Frontiers in Chemistry, 10:988587. https://doi.org/10.3389/fchem.2022.988587
RAPISARDA V., MIOZZI E., LORETO C. (2018) Cadmium exposure and prostate cancer: insights, mechanisms and perspectives. Front Biosci, 23(9):1687–1700. https://doi.org/10.2741/4667
SEILKOP S.K., OLLER A.R. (2003) Respiratory cancer risks associated with low-level nickel exposure: an integrated assessment based on animal, epidemiological, and mechanistic data. Regulatory toxicology and pharmacology, 37(2):173–190.
https://doi.org/10.1016/s0273-2300(02)00029-6
SEO C., LEE J.W., JEONG J.W., KIM T.S., LEE Y., GANG G., LEE S.G. (2023) Current technologies for heavy metal removal from food and environmental resources. Food science and biotechnology, 33(2):287–295. https://doi.org/10.1007/s10068-023-01431-w
SHETTY B.R., JAGADEESHA P.B., SALMATAJ S.A. (2025) Heavy metal contamination and its impact on the food chain: exposure, bioaccumulation, and risk assessment. CyTA - Journal of Food, 23(1):2438726. https://doi.org/10.1080/19476337.2024.2438726
SUMONA M., SOUMYADEEP M., MOHD A.H., BHASKAR S.G. (2015) Contemporary Environmental Issues of Landfill Leachate: Assessment and Remedies. Critical Reviews in Environmental Science and Technology, 45(5):472-590. https://doi.org/10.1080/10643389.2013.876524
SUSTAINABLE ENVIRONMENT RESEARCH INSTITUTE (2023) Study Project for Developing Public Policy on Waste Management System Reform and Promotion of Circular Economy (Phase 2). Chulalongkorn University, Bangkok, Thailand. (In Thai)
UGONNA C.N., PRECIOUS O.O., NNEKA I.O. (2020) A Review of the Health Implications of Heavy Metals in Food Chain in Nigeria. The Scientific World Journal, 2020:6594109.
https://doi.org/10.1155/2020/6594109
UNEP (2024) Beyond an age of waste Turning rubbish into a resource, UNEP, Nairobi, Kenya.
USEPA (1989) Risk assessment guidance for superfund. Human health evaluation manual (Part A) (Vol. 1). Office of Emergency and Remedial Response, Washington, DC, USA.
USEPA (1996) Microwave Assisted Acid Digestion of Siliceous and Organically Based Matrices, Method 3052. USEPA, Washington, DC, USA.
USEPA (2002a) Guidance on Choosing a Sampling Design for Environmental Data Collection. EPA QA/G-5S, USEPA, Washington, DC, USA.
USEPA (2002b) Treatment Technologies for Mercury in Soil, Waste, and Water. Office of Solid Waste and Emergency Response, Washington, DC, USA.
USEPA (2005) Guidelines for Carcinogen Risk Asses-sment. EPA/630/P-03/001F, Washington, DC, USA.
USEPA (2011) Exposure factors handbook 2011 edition). https://cfpub.epa.gov/ncea/risk/recordisplay.cfm?deid=236252.
USEPA (2012) Regional Screening Levels (Formerly PRGs)—Summary Table.
http://www.epa.gov/region9/superfund/prg.
USEPA (2013) Integrated risk information system (IRIS):Assessments. https://cfpub.epa.gov/ncea/risk/recordisplay.cfm?deid=2776.
USEPA (2018) Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES), Method 6010D, USEPA, Washington, DC, USA.
WHO/FAO (2002) Joint FAO/WHO General Standard for Contaminants and Toxins in Food and Feed, Shedule 1 Maximum and Guideline Levels for Contaminants and Toxins in Food. Rotterdam, The Nertherlands.
WHO/FAO (2007) Joint FAO/WHO Food Standard Programme Codex Alimentarius Commission on Food, 13th session. Houston, USA.
WHO/FAO (2011) Joint FAO/WHO Food Standard Programme Codex Alimentarius Commission on Food, Fifth Session. The Hague, The Netherlands.
WORLD BANK (2012) What A Waste: A global review of solid waste management. Washington, DC, USA.
WORLD BANK (2018) What a Waste 2.0: A Global Snapshot of Solid Waste Management to 2050. World bank, Washington, DC, USA.
YAN B., WEI Q., LI X., SONG X., GAO Z., LIU J., ZHANG R. WANG M. (2023) Heavy Metal Content Characteristics and Pollution Source Analysis of Shallow Groundwater in Tengzhou Coal Mining Area. Water, 15:4091. https://doi.org/10.3390/w15234091
YONG M.L., CUI L.Q., BING Z., HUI J. (2023) Development and validation of single-step microwave-assisted digestion method for determining heavy metals in aquatic products: Health risk assessment. Food Chemistry, 402:134500. https://doi.org/10.1016/j.foodchem.2022.134500
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