Elemental interactions in soil and their impact on soil fertility under the influence of treated wastewater and biosolid

Authors

  • Anastasia Kolokotsa Hellenic Open University, School of Science and Technologies, Greece
  • Prodromos Koukoulakis Agricultural Research Organization DEMETRA, Soil Science Institute, Thermi, Thessaloniki, Greece
  • Spyridon Kyritsis Hellenic Open University, School of Science and Technologies, Greece
  • Ioannis Kalavrouziotis Hellenic Open University, School of Science and Technologies, Greece

DOI:

https://doi.org/10.6092/issn.2281-4485/21827

Keywords:

heavy metals, Interactive behavior, Macronutrients interactions, Micronutrients interactions, Soil fertility

Abstract

Treated wastewater and biosolids are increasingly used in agriculture as alternatives to well irrigation water and as organic fertilizers. While these practices offer benefits, they also present challenges. Both treated wastewater and biosolids contain essential plant nutrients and heavy metals, which vary based on their source. When applied to soil, these elements interact with each other and with soil properties, potentially affecting soil fertility. To investigate these interactions, a greenhouse pot experiment was conducted using a simulated soil, based on a randomized block design with 12 treatment combinations, four replications, and 48 pots. The goal was to examine how heavy metals, nutrients and soil properties such as pH, clay content, organic matter, and electrical conductivity influence soil fertility when treated with wastewater and biosolids. The interactions were classified into six groups, and their nutrient contributions were analyzed using regression analysis. The study found several significant interactions, with the highest contributions observed in macronutrients, particularly from the interactions between “soil properties x macronutrients” and “micronutrients x macronutrients.” Significant contributions included nitrate (1016.05 mg/kg), potassium (783.07 mg/kg), calcium (4014.52 mg/kg), magnesium (475.46 mg/kg), and micronutrients like boron (2.02 mg/kg), zinc (11.98 mg/kg), copper (16.78 mg/kg), and iron (29.76 mg/kg).  The present study offers new insights into how elemental interactions affect soil fertility, under the influence of treated wastewater and biosolids. It highlights the importance of understanding these interactions for the effective management of the interactively contributed plant nutrients in the presence of the applied wastewater and biosolids.

References

ABEGUNRIN T.P., AWE G.O., IDOWU D.O., ADEJU-MOBI M.A. (2016) Impact of wastewater irrigation on soil physico-chemical properties, growth and water use pattern of two indigenous vegetables in southwest Nigeria. Catena 139: 167–178. https://doi.org/10.1016/j.catena.2015.12.014

ABOYEJI C.M., DUNSIN O., ADEKIYA A.O., SULEI-MAN K.O., CHINEDUM C., OKUNLOLA F.O., JOSEPH A., EJUE S.W., ADESOLA, O.O., OLOFIN-TOYE, T.A.J., OWOLABI I.O. (2020) Synergistic and antagonistic effects of soil applied P and Zn fertilizers on the performance, minerals and heavy metal composition of groundnut. Open Agriculture 5: 1–9. https://doi.org/doi:10.1515/opag-2020-0002

ABREU G.M., GUIRARDI B.D., PERES L.C., SOUZA J.R.M. DE SCHIAVO J.A. (2025) Effect of macronutrient deprivation on growth and nutrition of Jatropha curcas seedlings. Joutrnal Plant Nutririon, 1–12. https://doi.org/10.1080/01904167.2025.2467405

ALCALDE-SANZ L., GAWLIK B.. (2017) Minimum quality requirements for water reuse in agricultural irrigation and aquifer recharge - Towards a water reuse regulatory instrument at EU level. Publications Office of the European Union, Luxembourg, https://doi.org/10.2760/887727

AMEEN N., AMJAD M., MURTAZA B., ABBAS G., SHAHID M., IMRAN M., NAEEM M.A., NIAZI N.K. (2019) Biogeochemical behavior of nickel under different abiotic stresses: toxicity and detoxification mechanisms in plants. Environmental Science and Pollution Research In-ternational,. 26:10496–10514. https://doi.org/10.1007/s11356-019-04540-4

ANGELAKIS A.N., GIKAS P. (2014) Water reuse: Overview of current practices and trends in the world with emphasis on EU states. Water Utility Journal, 8: 67–78.https://doi.org/10.1117/12.740168

APHA (2017) Standard Methods for Examination of Water and Wastewater, American Public Health Association, AWWA, WEF, 22nd ed. American Public Health Association, Washington.

ARKOUN M., JANNIN L., LAÎNÉ P., ETIENNE P., MASCLAUX-DAUBRESSE C., CITERNE S., GARNICA M., GARCIA-MINA J.M., YVIN J.C., OURRY A. (2013) A physiological and molecular study of the effects of nickel deficiency and phenylphosphorodiamidate (PPD) applica-tion on urea metabolism in oilseed rape (Brassica napus L.). Plant Soil, 362: 79–92. https://doi.org/10.1007/s11104-012-1227-2

BASHOUR I., SAYEGH A.H. (2007) Methods of analysis for soils of arid and semi-arid regions. FAO, Rome. ISBN: 978-92-5-105661-5

CHAUHAN S.S., THAKUR R., SHARMA G.D. (2008) Nickel: Its availability and reactions in soil. Journal of Industrial Pollution Control, 24:57–62.

DEMITRELOS E., KOUKOULAKIS P.H., KYRITSIS S.S., BOUNTLA A., ISARI E., KALAVROUZIOTIS I.K. (2022) Impact of Elemental Interactions on Soil Metal Availability and Growth of Ryegrass (Lolium perenne L.) Under Wastewater and Biosolids. Water, Air, Soil Pollution, 233: 96. https://doi.org/10.1007/s11270-022-05561-5

GEE G.W., BAUDER J.W. (2018) Particle-size Analysis, in: Klute, A.B.T.-S.B.S., Klute, A. (Eds.), SSSA Book Series. Madison, WI, USA, pp. 383–411.https://doi.org/10.2136/sssabookser5.1.2ed.c15

IYAKA Y.A. (2011) Nickel in soils: A review of its distribution and impacts. Scientific Research and Essays, 6:6774–6777. https://doi.org/10.5897/SREX11.035

KALAVROUZIOTIS I.K., KOUKOULAKIS P.H., RO-BOLAS P., PAPADOPOULOS A.H., PANTAZIS V., (2008) Interrelationships of heavy metals macro and micro- nutrients, and properties of a soil cultivated with Brassica oleracea var. italica (Broccoli), under the effect of treated municipal wastewater. Water. Air. Soil Pollution. 190:309–321. ttps://doi.org/10.1007/s11270-007-9602-y

KICIŃSKA A., POMYKAŁA R., IZQUIERDO M. (2022) Changes in soil pH and mobility of heavy metals in contaminated soils. European Journal of Soil Science, 73:e13203. https://doi.org/10.1111/ejss.13203

KOUKOULAKIS P.H., KANATAS P., KYRITSIS S.S., NTZALA G., KALAVROUZIOTIS I.K. (2023) The impact of the elemental interactions on soil fertility and toxicity in the presence of wastewater and biosolids: A quantitative evaluation. Water 15: 3743. https://doi.org/10.3390/w15213743

KUBIER A., WILKIN R.T., PICHLER T. (2019) Cad-mium in soils and groundwater: A review. Applied Geochemistry, 108: 104388. ttps://doi.org/10.1016/j.apgeochem.2019.104388

LINDSAY W.L., NORVELL W.A. (1978) Development of a DTPA Soil Test for Zinc, Iron, Manganese, and Copper. Soil Science Society of America Journal, 42: 421–428. https://doi.org/10.2136/sssaj1978.03615995004200030009x

MAGDOFF F.R., JOKELA W.E., FOX R.H., GRIFFIN G.F. (1990) A soil test for nitrogen availability in the northeastern United States. Communications in Soil Science and Plant Analysis, 21: 1103–1115. https://doi.org/10.1080/00103629009368293

MEHNAZ K.R., KEITEL C., DIJKSTRA F.A. (2019) Phosphorus availability and plants alter soil nitrogen retention and loss. Science of The Total Environment, 671: 786–794. https://doi.org/https://doi.org/10.1016/j.scitotenv.2019.03.422

MENGE K., KIRKBY E.A., KOSEGARTEN H., AP-PEL T. (2001) Plant Nutrients BT - Principles of Plant Nutrition, in: Mengel, K., Kirkby, E.A., Kosegarten, H., Appel, T. (Eds.), . Springer Netherlands, Dordrecht, pp. 1–13. https://doi.org/10.1007/978-94-010-1009-2_1

MILLER J., CURTIN D. (2007) Electrical Conductivity and Soluble Ions, in: Carter M., Gregorich E. Second Edition, E.B.T.-S.S. and M. of A., Carter M., Gregorich, E. (Eds.), Soil Sampling and Methods of Analysis, Second Edition. https://doi.org/10.1201/9781420005271.ch15

NOULAS C., TZIOUVALEKAS M., KARYOTIS T., (2018) Zinc in soils, water and food crops. Journal of Trace Elements in Medicine and Biology, 49: 252–260. https://doi.org/10.1016/j.jtemb.2018.02.009

OLSEN S.R., COLE C.V., WATANABE F.S., DEAN L.A. (1954) Estimation of available Phosphorus in soils by extraction with Sodium Bicarbonate. Cir. No 939, USDA, Washington.

RENGEL Z. (2004) Heavy Metals as Essential Nutrients, in: Prasad, M.N. V (Ed.), Heavy Metal Stress in Plants. Springer Berlin Heidelberg, Berlin, Heidelberg, pp. 271–294. https://doi.org/10.1007/978-3-662-07743-6_11

ROIG N., SIERRA J., MARTÍ E., NADAL M., SCHUH-MACHER M., DOMINGO J.L. (2012) Long-term amend-ment of Spanish soils with sewage sludge : Effects on soil functioning. Agriculture, Ecosystems and Environment, 158:41–48. https://doi.org/10.1016/j.agee.2012.05.016

SESHADRI B., BOLAN N.S., WIJESEKARA H., KUN-HIKRISHNAN A., THANGARAJAN R., QI F., MA-THEYARASU R., ROCCO C., MBENE K., NAIDU R. (2016) Phosphorus-cadmium interactions in paddy soils. Geoderma, 270: 43–59. https://doi.org/10.1016/j.geoderma.2015.11.029

SHABA P.J., MUSA D.A., EGWIM E.C., UTHMAN A., (2022) Function of Urease in Plants with Reference to Legumes: A Review, in: Jimenez-Lopez, J.C., Clemente, A. (Eds.). IntechOpen, Rijeka. https://doi.org/10.5772/intechopen.102646

SHEN Z., CHEN Y., XU D., LI L., ZHU Y. (2020) Interactions between heavy metals and other mineral elements from soil to medicinal plant Fengdan (Paeonia ostii) in a copper mining area, China. Environmental Science and Pollution Research, 27:33743–33752. https://doi.org/10.1007/s11356-020-09358-z

STRAWN D.G. (2021) Sorption mechanisms of chemicals in soils. Soil Systems, 5: 1–22.https://doi.org/:10.3390/soilsystems5010013

SUDA A., MAKINO T. (2016) Functional effects of manganese and iron oxides on the dynamics of trace elements in soils with a special focus on arsenic and cadmium: A review. Geoderma 270: 68–75. https://doi.org/10.1016/j.geoderma.2015.12.017

SUMNER M.E., MILLER W.P. (2018) Cation exchange capacity and exchange coefficients, In: Sparks D.L., Page A.L., Helmke P.A., Loeppert R.H., Soltanpour P.N., Tabatabai M.A., Johnston C.T., Loeppert R.H., Soltanpour P.N., Tabatabai M.A., Johnston C.T., Sumner M.E. (Eds.) SSSA Book Series. Madison, WI, USA, pp. 1201–1229. https://doi.org/10.2136/sssabookser5.3.c40

TKALEC M., STEFANIĆ P.P., CVJETKO P., SIKIĆ S., PAVLICA M., BALEN B. (2014) The effects of cadmium-zinc interactions on biochemical responses in tobacco seedlings and adult plants. PLoS One 9: e87582–e87582. https://doi.org/10.1371/journal.pone.0087582

TRIPATHI D.K., SINGH V.P., CHAUHAN D.K., PRA-SAD S.M., DUBEY N.K. (2014) Role of Macronutrients in Plant Growth and Acclimation: Recent Advances and Fu-ture Prospective BT-Improvement of Crops in the Era of Climatic Changes: Volume 2, in: Ahmad, P., Wani, M.R., Azooz, M.M., Phan Tran, L.-S. (Eds.), Springer New York, 197–216. https://doi.org/10.1007/978-1-4614-8824-8_8

UMAR A.W., NAEEM M., HUSSAIN H., AHMAD N., XU M. (2025) Starvation from within: How heavy metals compete with essential nutrients, disrupt metabolism, and impair plant growth. Plant Science, 353: 112412. https://doi.org/10.1016/j.plantsci.2025.112412

XIE K., CAKMAK I., WANG S., ZHANG F., GUO S., (2021) Synergistic and antagonistic interactions between potassium and magnesium in higher plants. The Crop Jour-nal, 9:249–256. https://doi.org/10.1016/j.cj.2020.10.005

YAN B., SUN Y.Y., WEI Y. (2020) Potassium–calcium an- tagonistic interaction under tomato magnesium deficiency and magnesium fertiliser regulation in solar greenhouse. Quality Assurance and Safety of Crops & Foods, 12:76–86. https://doi.org/10.15586/qas.v12i3.723

ZHANG L., XIONG K., WAN P. (2023) Effects of Heavy Metals on Nitrogen in Soils of Different Ecosystems in the Karst Desertification of South China. Forests, 14. https://doi.org/10.3390/f14071497

ZHU Z., ZHANG H., TIAN H., CHAI G., MUHAM-MAD R., WANG Q., LIANG B., WU X. (2025) Compre-hensive Analysis of Magnesium Deficiency Effects on Photosynthesis and Energy Balance in Tomato Leaves. Plant Physiology and Biochemistry, 109671.https://doi.org/10.1016/j.plaphy.2025.109671

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Published

2025-06-16

How to Cite

Kolokotsa, A., Koukoulakis, P., Kyritsis, S., & Kalavrouziotis, I. (2025). Elemental interactions in soil and their impact on soil fertility under the influence of treated wastewater and biosolid. EQA - International Journal of Environmental Quality, 70, 11–27. https://doi.org/10.6092/issn.2281-4485/21827

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