Use of Laurus nobilis (Noble Bay) essential oil as a preservative for a meat product, Mortadella
DOI:
https://doi.org/10.60923/issn.2281-4485/22528Keywords:
Preservative, extraction, essential oils, Laurus nobilis, antimicrobial activityAbstract
Our objective is to find a natural preservative for a food product as an alternative to prohibited chemical substances. The essential oils from the leaves of Laurus nobilis harvested in the Relizane region were characterized by physicochemical methods followed by gas chromatography coupled with a mass spectrometer detector. The effectiveness of the extracted oils was tested against microbial strains by the agar diffusion method. Indeed, two doses were incorporated into the mortadella (5μL and 10μL). The extraction yield was equal to 0.95 ± 0.45%, with values of refractive index (1.3138 ± 0.25), acid index (0.225 ± 0.001), saponification index (31.5 ± 0.005 (mg KoH/g), ester index (31.25 ± 0.05) and relative density (0.825 ± 0.001). Chromatographic analyses showed the predominance of eucalyptol (29.79%). The antimicrobial activity test of the essential oil showed a potent activity against Staphylococcus aureus (80 mm) and Pseudomonas aeruginosa (30 mm). At T20, no growth of total mesophilic aerobic flora and pathogenic germs was observed in samples containig Essential oils.
References
AFNOR (Association Française pour la Normalisation). (1984) Recueil des normes françaises : corps gras, graines oléagineuses et produits dérivés (3rd eds.). AFNOR., Paris.
SAHIN BASAK S., CANDAN F. (2013) Effect of Laurus nobilis L. essential oil and its main components on α-glucosidase and reactive oxygen species scavenging activity. Iranian Journal
BELLETTI N., NDAGIJIMANA M., SISTO C., GUER-ZONI M.E., LANCIOTTI R., GARDINI F. (2004) Evalua-tion of the antimicrobial activity of citrus essences on Saccharomyces cerevisiae. Journal Agricultural and Food Chemi-stry, 52(23):6932-8. https://doi.org/10.1021/jf049444v
BOUHDID S., ABRINI J., ZHIRI A., ESPUNY M.J., MANRESA A. (2009) Investigation of functional and morpho-logical changes in Pseudomonas aeruginosa and Staphylococcus aureus cells induced by Origanum compactum essential oil. Journal of Applied Microbiology, 106(5):1558-1568. https://doi.org/10.1111/j.1365-2672.2008.04124.x
BOUKHENNOUFA A., TIR TOUIL MEDDAH A., MEDDAH B., GABALDON J.A., SONNET P.(2019) Comparative study of Artemisia herba alba asso and Citrus aurantium essential oils. Journal of Microbiology Biotechnology and Food Sciences, 9(3): 622-627. https://doi.org/10.15414/jmbfs.2019/20.9.3.622-627
BRAHIMI D., MESLI L., RAHMOUNI A., ZEGGAI F., KHALDOUN B., CHEBOUT R., BELBACHIR M. (2020) First data of statistic and ecological behavior of Orthoptera insects in arid region (Southern West of Algeria). Data in Brief, 31: 105857. https://doi.org/10.1016/j.dib.2020.105857
BURT S. (2004) Essential oils: their antibacterial properties and potential applications in foods. a review. International Journal of Food Microbiology, 94: 223–253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
COWAN M.M. (1999) Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12:564-582. https://doi.org/10.1128/CMR.12.4.564
DENIS J., BRIANT J., HIPEAUX J.C. (1997) Physico-Chimie des Lubrifiants. Analyses et essais. Ophrys., Paris. ISBN/ISSN/EAN : 978-2-7108-0726-1.
DJENANE D., AÏDER M., YANGÜELA J., IDIR L., GOMEZ D., RONCALES P. (2012) Antioxidant and antibacterial effects of Lavandula and Mentha essential oils in minced beef inoculated with E. coli O157:H7 and S. aureus during storage at abuse refrigeration temperature. Meat Science, 92(4):667-674. https://doi.org/10.1016/j.meatsci.2012.06.019
GIOVANELLI S., CICCARELLI D., GIUSTI G., MAN-CIANTI F., NARDONI S., PISTELLI L. (2020) Compara-tive assessment of volatiles in juices and essential oils from minor Citrus fruits (Rutaceae). Flavour and Fragrance Journal,10:1–14. https://doi.org/10.1002/ffj.3603
HYLDGAARD M., MYGIND T., MEYER, R.L. (2012) Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Frontiers in microbiology, 3(12): 1-24. https://doi.org/10.3389/fmicb.2012.00012
ELA M.A., EL-SHAER N.S., GHANEM N.B. (1996) Anti-microbial evaluation and chromatographic analysis of some essential and fixed oils. Pharmazie, 51: 993- 995. https://pubmed.ncbi.nlm.nih.gov/8985990/
NOVIDZRO K.M., WOKPOR K., AMOUSSOU F.B., KOUDOUVO K., KOKOUVI D.K., OSSEYI E., KOUMAGLO K.H. (2019) Etude de quelques paramètres physicochimiques et analyse des éléments minéraux, des pigments chlorophylliens et caroténoïdes de l’huile de graines de Griffonia simplicifolia. International journal of Biological and Chemical Sciences, 13(4): 2360-2373. https://dx.doi.org/10.4314/ijbcs.v13i4.38
OULED TAARABT K., KOUSSA T., ALFEDDY M.N. (2017) Caractéristiques physicochimiques et activité antimicrobienne de l’huile essentielle du Laurus nobilis L. au Maroc. Afrique. Science, 13(1): 349–359. ISSN: 1813-548X.
OULD YEROU K., MEDDAH B., TIR TOUIL A. (2015) Etude de l’effet d’huile essentielle de laurier Noble de l’ouest Algérien sur Salmonella Spp. in vitro et in Vivo. European Scientific Journal, 11(33). ISSN: 1857-7881.
RAO U.M, SOOD Y.R., JARIAL R.K. (2016) Ester Dielectrics: Current Perspectives and Future Challenges. IETE Technical Review, 448-459. https://doi.org/10.1080/02564602.2016.1202088
SINDELAR J.J., MILKOWSKI A.L. (2012) Human safety controversies surrounding nitrate and nitrite in the diet. Nitric Oxide, 26(4): 259-266. https://doi.org/t10.1016/j.niox.2012.03.011
TAJKARIMI M.M., IBRAHIM S.A., CLIVER D.O. (2010) Antimicrobial herb and spice compounds in food. Food Control, 21(9):1199-1218. https://doi.org/10.1016/j.foodcont.2010.02.003.
WAGNER H., BLADT S., (1996) Essential Oil Drugs, In: Plant drug analysis. A thin layer chromatography atlas. Springer., Berlin, Germany. https://doi.org/10.1177/002580248502500314
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Copyright (c) 2026 Asma Boukhennoufa , Ibtissem Fergane , Nour El Houda Fergane , Boumediene Meddah , Aicha Meddah Tir Touil, Fayssal Abdelaaziz Bouali Youcef

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