Assessment of Heavy Metals contamination in Cannabis sativa, soil and industrial wastewater from Mirpur (Azad Jammu and Kashmir)
DOI:
https://doi.org/10.60923/issn.2281-4485/24209Keywords:
Heavy metals, Cannabis sativa, soil, waste water, MirpurAbstract
Industrial effluent–derived heavy metal contamination poses serious ecological and human health risks due to persistence, toxicity, and bioaccumulation in soil–plant–water systems. This study assessed the distribution, bioaccumulation, and ecological risk of selected heavy metals in Cannabis sativa, soil and water from an industrial area of Mirpur, Azad Jammu and Kashmir (AJK). Cannabis sativa and soil samples were collected from three locations at increasing distances from the industrial zone, while water samples were obtained from steel, textile, pharmaceutical, and combined industrial effluents. After acid digestion, concentrations of zinc (Zn), manganese (Mn), copper (Cu), cobalt (Co), nickel (Ni), cadmium (Cd), tin (Sn), and silver (Ag) were measured using atomic absorption spectrophotometry and compared with WHO permissible limits. Soil-to-plant transfer and water contamination patterns were evaluated to infer ecological and bioaccumulation risk. Results revealed elevated metal levels across all matrices. In C. sativa, mean concentrations of Zn (3.62 ppm), Mn (3.48 ppm), Cu (2.44 ppm), Co (2.33 ppm), Ni (1.23 ppm), Cd (2.16 ppm), Sn (1.21 ppm), and Ag (1.54 ppm) exceeded WHO limits. Soil concentrations were higher, e.g., Zn (13.09 ppm), Mn (16.34 ppm), Co (10.45 ppm), Ni (3.90 ppm), Cd (3.13 ppm). Water samples exhibited the highest concentrations in mixed effluents: Co (8.67 ppm), Ni (13.22 ppm), Mn (9.73 ppm), Zn (11.23 ppm), Cu (3.99 ppm), Cd (6.23 ppm), Ag (1.12 ppm), and Sn (2.02 ppm). Cadmium, cobalt, and zinc showed higher bioaccumulation potential in plants. Overall, contamination and bioaccumulation indices indicate moderate to high ecological risk, highlighting the need for continuous monitoring and stricter regulation of industrial effluent discharge in Mirpur AJK.
References
ADAMS I.B., MARTIN B.R. (1996) Cannabis: Pharmaco-logy and Toxicology in Animals and Humans. Addiction, 91: 1585-1514. https://doi.org/10.1046/j.1360-0443.1996.911115852.x
AHMED M., MATSUMOTO M., OZAKI A., THINH N. V., KUROSAWA K. (2019) Heavy Metal Contamination of Irrigation Water, Soil, and Vegetables and the Difference between Dry and Wet Seasons Near a Multi-Industry Zone in Bangladesh. Water, 11(3): 583. https://doi.org/10.3390/w11030583
AKPOR O.B., MUCHIE M. (2011) Environmental and public health implications of wastewater quality. African Journal of Biotechnology, 10(13): 2379-2387. https://doi.org/10.5897/AJB10.1797
ALLOWAY B.J. (2013). Heavy Metals in Soils: Trace Metals and Metalloids in Soils and their Bioavailability. Third edition Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4470-7
ASIMINICESEI D.M., VASILACHI I.C., MARIA GAVRILESCU M. 2020 Heavy Metal Contamination of Medicinal Plants and Potential Implications on Human Health. Revista de Chimie, 71 (7):16-36. https://doi.org/10.37358/RC.20.7.8222
BRADL H.B, (2004) Adsorption of heavy metal ions on soils and soils constituents. Journal of Colloid and Interface Science, 277(1):1-18. https://doi.org/10.1016/j.jcis.2004.04.005
BRÜMMER G.W., GERTH J., HERMS, U. (1986) Heavy Metal Species, Mobility and Availability in Soils. Zeitschrift für Pflanzenernährung und Bodenkunde, 149: 382-398.
https://doi.org/10.1002/jpln.19861490404
CHANDRA S., (2017) Phytochemistry of Cannabis sativa L. Progress in the chemistry of Organic Natural Products. https://doi.org/10.1007/978-3-319-45541-9_1
FU F., WANG Q. (2011) Removal of heavy metal ions from wastewaters: A review. Journal of Environmental Management, 92(3): 407–418. https://doi.org/10.1016/j.jenvman.2010.11.011
KABATA-PENDIAS A. (2010) Trace Elements in Soils and Plants, (4th ed.). CRC Press. https://doi.org/10.1201/b10158
LYNCH, M.E. AND CAMPBELL, F. (2011) Cannabinoids for treatment of chronic non-cancer pain; a systematic re-view of randomized trials. British Journal of Clinical Pharma-cology, 72: 735-744. https://doi.org/10.1111/j.1365-2125.2011.03970.x
MAHMOOD A, RASHID S, MALIK R.N. (2013) Determi-nation of toxic heavy metals in indigenous medicinal plants used in Rawalpindi and Islamabad cities, Pakistan. Journal of Ethnopharmacology, 148(1):158-164. https://doi.org/10.1016/j.jep.2013.04.005
MIRETZKY P., SARALEGUI A., CIRELLI A. F. (2004) Aquatic macrophytes potential for the simultaneous removal of heavy metals (Buenos Aires, Argentina). Chemosphere, 57 (8):997–1005. https://doi.org/10.1016/j.chemosphere.2004.07.024
NAGAJYOTI, P.C., LEE, K.D., SREEKANTH, T.V.M. (2010) Heavy metals, occurrence and toxicity for plants: A review. Environmental Chemistry Letters, 8: 199–216. https://doi.org/10.1007/s10311-010-0297-8
ODIEKA A.E., OBUZOR G.U., OYEDEJI O.O., GONDWE M., HOSU Y.S., OYEDEJI A.O. (2022) The Medicinal Natural Products of Cannabis sativa Linn.: A Review. Molecules, 27(5): 1689. https://doi.org/10.3390/molecules27051689
SABIR, M., BALTRĖNAITĖ-GEDIENĖ, E., DITTA, A., ULLAH, H., KANWAL, A., ULLAH, S., & FARAJ, T. K. (2022) Bioaccumulation of Heavy Metals in a Soil–Plant System from an Open Dumpsite and the Associated Health Risks through Multiple Routes. Sustainability, 14(20): 13223. https://doi.org/10.3390/su142013223
TCHOUNWOU PB, YEDJOU CG, PATLOLLA AK, SUTTON DJ. (2012) Heavy metal toxicity and the environment. Experientia Supplementum, 101:133-64. https://doi.org/10.1007/978-3-7643-8340-4_6
TÓTH G., HERMANN T., DA SILVA M.R., MONTANARELLA L. (2016) Heavy metals in agricultural soils of the European Union with implications for food safety-Environment international, 88: 299–309. https://doi.org/10.1016/j.envint.2015.12.017
WANG S., GUO Y., HU H., LIANG Y., LI K., ZHANG K., HOU G., LI C., ZHANG J., WANG,Z. (2025). Sources, distribution, and health risks of heavy metal contamination in the tongren mercury mining area: A case study on mercury and cadmium. toxics. 13(7): 527. https://doi.org/10.3390/toxics13070527
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Asia Bostan, Muhammad Alamzeb, Saqib Ali

This work is licensed under a Creative Commons Attribution 4.0 International License.

