Preliminary Observations On The Metal Tolerance And Resilience Capacity Of Helichrysum Italicum (Roth) G. Don Growing On Mine Soils

Claudio Bini, Laura Maleci, Corrado Tani, Mohammad Wahsha

Abstract


Wild specimens of Helichrysum italicum, with their soil clod, were gathered from sites with different contamination levels by heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn) in the abandoned Niccioleta mine (Tuscany, Italy). We examined the effects of heavy metals (HM) on the morphology of H. italicum growing on mine soils, with the following objectives: to determine the fate of HM within the soilplant system; to highlight morphological modifications at anatomical and cytological level; to ascertain the plant tolerance to heavy metals, and their resilience capacity. Plants appeared macroscopically not affected by toxic signals.
Light microscopy and TEM observations of leaves show a clear difference in the cell organization of not-contaminated and contaminated samples. Trichomes of the polluted plants present a completely different morphology in comparison to nonpolluted ones, with a stalk of 3-4 cells and a large secreting apical cell, a palisade parenchyma less organized, and a reduction of leaf thickness. A gradual restoration of cell organization suggests that somewhat resilience occurred in plants. The resilience capacity points to H. italicum as an useful species in remediation projects.

Keywords


heavy metals; mine soils; plant morphology; Helichrysum italicum; ultrastructure

Full Text:

PDF (English)

References


BIANCHINI, A., SANTONI, F., PAOLINI, J., BERNARDINI, A.F., MOUILLOT,

D., COSTA, J. (2009) Partitioning the relative contributions of inorganic plant composition and soil characteristics to the quality of Helichrysum italicum subsp. italicum (Roth) G. Don essential oil. Chemistry & Biodiversity, 6:1014-1033.

BINI C. (2009) Soil restoration: remediation and valorization of contaminated soils. In: E.A.C. Costantini (ed.) Manual of methods for soil and land evaluation. Science Publisher, Enfield (NH), pp 137-160.

BINI C. (2012) Environmental impact of abandoned mine waste: a review. Nova Science, New York, pp. 92.

BINI C., WAHSHA M., FONTANA S., MALECI L. (2012) Effect of heavy metals

on morphological characteristics of Taraxacum officinale Web growing on mine soils in NE Italy. J Geochem Explor., 123:101–108

BINI C., BUFFA G., FONTANA S., WAHSHA M. (2013) - Are plants growing at

abandoned mine sites suitable for phytoremediation of contaminated soils?

Geophysical Research Abstracts Vol. 15, EGU2013-2123.

BULLITTA S., PILUZZA G., USAI M. (2010) Determinazione di metalli pesanti

accumulati in Helichrysum italicum (Roth) G. Don subsp. microphyllum (Willd.) Nyman vegetante su aree minerarie dismesse. Fitomed 2010, 116, Cagliari.

KUPPER H, KUPPER F, SPILLER M (1998) In situ detection of heavy metal

substituted chlorophylls in water plants. Photosynth Res., 58(2):123–133.

LATTANZI P., TANELLI G. (1985) Le mineralizzazioni a pirite ossidi di Fe e PbZn(Ag) della zona di Niccioleta (Grosseto). Rendiconti della Società italiana di mineralogia e petrologia, 40(2):385-408.

MALECI L., BUFFA G., WAHSHA M., BINI C. (2014) .Morphological changes

induced by heavy metals in dandelion (Taraxacum officinale Web.) growing on mine soils. J. Soils Sediments, 14:731–743.

MARGESIN R., SCHINNER F. (2005) Manual for soil analysis, monitoring and

assessing soil bioremediation, 1st edn. Springer, Berlin, Germany, p. 359.

McGRATH S. (1998) Phytoextraction for soil remediation. In: Plants that

hyperaccumulate heavy metals (R. Brooks ed.) CAB International, U.K., 261-287.

RASCIO N., NAVARI-IZZO F. (2011) Heavy metal hyperaccumulating plants: how and why do they do it? And what makes them so interesting? Plant Sci., 180: 169–181.

WAHSHA M., BINI C., FONTANA S., ZILIOLI D., WAHSHA A. (2012a)

Toxicity assessment of contaminated soils from a mining area in northeast Italy by using lipid peroxidation assay. J Geochem Explor., 113:112-117.

WAHSHA M, BINI C, ARGESE E, MINELLO F, FONTANA S, WAHSHEH H

(2012b) Heavy metals accumulation in willows growing on Spolic Technosols from the abandoned Imperina Valley mine in Italy. J Geochem Explor., 123:19–24.

ZHAO S, YE X, ZHENG J (2011) Lead-induced changes in plant morphology, cell ultrastructure, growth and yields of tomato. Afr. J. Biotechnol., 10(50):10116– 10124.




DOI: 10.6092/issn.2281-4485/6599

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Claudio Bini, Laura Maleci, Corrado Tani, Mohammad Wahsha

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License.

Creative Commons License

Online ISSN 2281-4485

The journal is hosted and mantained by ABIS-AlmaDL. [privacy]